Hotspot

trnM-CAU-atpE

LSC · 55355-55632 · 278 bp

Back to Pristimera

Pi 0.0566

MarkerSeek score 83.50

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 23
pi: 0.0566
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	376-381 bp	1.0	84.9
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	TTGAGGCAAATCTCGCTCTC	50.0	50.0	58.4	58.0	391-396 bp	1.0	84.7
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	58.4	58.0	403-408 bp	1.0	84.6
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	TCTCAGACGAGCTAGGACAC	50.0	55.0	58.4	58.3	375-380 bp	1.0	83.1
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	57.3	58.0	404-409 bp	1.0	82.6