Hotspot

trnF-GAA-ndhJ

LSC · 51276-52128 · 853 bp

Back to Pristimera

Pi 0.0233

MarkerSeek score 71.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 28
pi: 0.0233
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	965-1001 bp	1.0	86.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	964-1000 bp	1.0	86.0
trnF-GAA-ndhJ_p3	GCATTGAGAATGGTCGGGAT	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.0	58.0	980-1016 bp	1.0	85.7
trnF-GAA-ndhJ_p4	GCATTGAGAATGGTCGGGAT	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.0	58.0	979-1015 bp	1.0	85.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	928-964 bp	1.0	85.6