Hotspot

rpoB-trnC-GCA

LSC · 27482-28497 · 1016 bp

Back to Pristimera

Pi 0.0216

MarkerSeek score 67.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 30
pi: 0.0216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTACATCCCCCAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1030-1100 bp	1.0	85.5
rpoB-trnC-GCA_p2	CCTCATTTACATCCCCCAGC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1012-1082 bp	1.0	83.3
rpoB-trnC-GCA_p3	TCCCTCATTTACATCCCCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1032-1102 bp	1.0	83.2
rpoB-trnC-GCA_p4	TCCCTCATTTACATCCCCCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.7	58.4	1014-1084 bp	1.0	81.1
rpoB-trnC-GCA_p5	CCCTCATTTACATCCCCCAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.3	58.1	1031-1101 bp	1.0	80.3