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trnH-GUG-psbA

LSC · 67-736 · 670 bp

Back to Pristimera

Pi 0.0866

MarkerSeek score 81.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 670
variable_sites: 66
pi: 0.0866
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTGTCGAAACTCCATCCACA	55.0	50.0	58.1	57.5	585-721 bp	1.0	87.3
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ACTGTCGAAACTCCATCCAC	55.0	50.0	58.1	57.5	586-722 bp	1.0	87.3
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CTGTCGAAACTCCATCCACA	50.0	50.0	57.9	57.5	599-735 bp	1.0	87.3
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CTGTCGAAACTCCATCCACA	50.0	50.0	57.9	57.5	598-734 bp	1.0	87.3
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CTGTCGAAACTCCATCCACA	50.0	50.0	57.9	57.5	600-736 bp	1.0	87.3