Hotspot

trnN-GUU-trnR-ACG

IRa · 136113-136967 · 855 bp

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Pi 0.0437

MarkerSeek score 71.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 855
variable_sites: 31
pi: 0.0437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	802-947 bp	1.0	83.9
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	GAACAGATCCAGTGGAGACG	50.0	55.0	58.2	57.7	839-984 bp	1.0	83.2
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	TTTTAGGGTACTCCGGGGAA	50.0	50.0	58.2	57.7	856-1001 bp	1.0	82.7
trnN-GUU-trnR-ACG_p4	TGGTCGTAGGTTCGAATCCT	TTTTTAGGGTACTCCGGGGA	50.0	50.0	58.2	57.7	857-1002 bp	1.0	82.7
trnN-GUU-trnR-ACG_p5	GGTTCGAATCCTACTTGGGG	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.7	57.8	794-939 bp	1.0	82.5