Hotspot

rpl32-trnL-UAG

SSC · 118850-120213 · 1364 bp

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Pi 0.0436
MarkerSeek score 76.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1364
variable_sites
57
pi
0.0436
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGAACTCAAAAAGCTTTTT AGCGTGTCTACCGATTTCAC 35.0 50.0 52.7 58.0 1441-1472 bp 1.0 46.3
rpl32-trnL-UAG_p2 GGGAACTCAAAAAGCTTTTT CAGCGTGTCTACCGATTTCA 35.0 50.0 52.7 58.0 1442-1473 bp 1.0 46.3
rpl32-trnL-UAG_p3 GGGAACTCAAAAAGCTTTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 52.7 57.9 1451-1482 bp 1.0 46.2
rpl32-trnL-UAG_p4 GGGAACTCAAAAAGCTTTTT CCTAAGAGCAGCGTGTCTAC 35.0 55.0 52.7 57.8 1450-1481 bp 1.0 46.2
rpl32-trnL-UAG_p5 GGGAACTCAAAAAGCTTTTT CTAAGAGCAGCGTGTCTACC 35.0 55.0 52.7 57.8 1449-1480 bp 1.0 46.2