Hotspot

trnR-ACG-trnN-GUU

IRb · 113444-114298 · 855 bp

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Pi 0.0437

MarkerSeek score 71.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 855
variable_sites: 31
pi: 0.0437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	802-947 bp	1.0	83.9
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	839-984 bp	1.0	83.2
trnR-ACG-trnN-GUU_p3	TTTTAGGGTACTCCGGGGAA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	57.7	58.2	856-1001 bp	1.0	82.7
trnR-ACG-trnN-GUU_p4	TTTTTAGGGTACTCCGGGGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	57.7	58.2	857-1002 bp	1.0	82.7
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GGTTCGAATCCTACTTGGGG	55.0	55.0	57.8	57.7	794-939 bp	1.0	82.5