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rps3-rps19

LSC · 88464-89214 · 751 bp

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Pi 0.0485

MarkerSeek score 80.90

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 36
pi: 0.0485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	TCTAAACTTTCGGGGACACG	50.0	50.0	57.5	57.6	840-897 bp	1.0	76.9
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	TCTAAACTTTCGGGGACACG	50.0	50.0	58.6	57.6	834-891 bp	1.0	76.0
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	TCTAAACTTTCGGGGACACG	45.0	50.0	57.0	57.6	878-935 bp	1.0	72.7
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	TCTAAACTTTCGGGGACACG	45.0	50.0	57.0	57.6	833-890 bp	1.0	72.2
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	TAAACTTTCGGGGACACGC	50.0	52.6	57.5	58.1	838-895 bp	1.0	71.8