Hotspot

psbF-psbE

LSC · 68687-68695 · 9 bp

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Pi 0.111
MarkerSeek score 75.60
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 TGGATAGGTTCTATCTATGGTCA GGCCGTTTTGATCCTTTGGA 39.1 50.0 55.5 58.7 84 bp 1.0 41.0
psbF-psbE_p2 TGGATAGGTTCTATCTATGGTCA GCCGTTTTGATCCTTTGGAA 39.1 45.0 55.5 56.9 83 bp 1.0 41.0
psbF-psbE_p3 TTGGATAGGTTCTATCTATGGTCA GGCCGTTTTGATCCTTTGGA 37.5 50.0 56.2 58.7 85 bp 1.0 40.9
psbF-psbE_p4 CCATCGTACTGTAAAAATTGGA GGCCGTTTTGATCCTTTGGA 36.4 50.0 54.5 58.7 102 bp 1.0 40.8
psbF-psbE_p5 CCATCGTACTGTAAAAATTGGA GCCGTTTTGATCCTTTGGAA 36.4 45.0 54.5 56.9 101 bp 1.0 40.8