Hotspot

trnF-GAA-ndhJ

LSC · 52693-53439 · 747 bp

Back to Prioria

Pi 0.0245

MarkerSeek score 74.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 18
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	885-898 bp	1.0	80.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	849-862 bp	1.0	79.7
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	57.9	57.7	894-907 bp	1.0	79.3
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	873-886 bp	1.0	78.9
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCCGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	883-896 bp	1.0	77.6