Hotspot

trnT-UGU-trnL-UAA

LSC · 50831-51485 · 655 bp

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Pi 0.06

MarkerSeek score 88.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 38
pi: 0.06
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 88.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	712-744 bp	1.0	85.0
trnT-UGU-trnL-UAA_p2	TAGGGGAAAAGAAAAGCCGG	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	769-801 bp	1.0	83.9
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	744-776 bp	1.0	83.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	718-750 bp	1.0	83.6
trnT-UGU-trnL-UAA_p5	TAGGGGAAAAGAAAAGCCGG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	775-807 bp	1.0	82.5