Hotspot

psbC-trnS-UGA

LSC · 38886-39119 · 234 bp

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Pi 0.0641

MarkerSeek score 73.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 15
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	58.4	58.2	330 bp	1.0	78.9
psbC-trnS-UGA_p2	GCTGCAGCAGGATTTGAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	329 bp	1.0	78.4
psbC-trnS-UGA_p3	TAGGTCATTTATGGCACGCG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.4	58.2	364 bp	1.0	78.2
psbC-trnS-UGA_p4	GTAGGTCATTTATGGCACGC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.0	58.2	365 bp	1.0	73.2
psbC-trnS-UGA_p5	CAGCTGCAGCAGGATTTGAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	59.1	58.2	331 bp	1.0	73.0