Hotspot

atpF-atpH

LSC · 13666-14563 · 898 bp

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Pi 0.0584

MarkerSeek score 73.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 38
pi: 0.0584
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCCAAAGAAAGGAAAGAATCGG	AGGCAGAGGGAAAAATACGG	45.5	50.0	57.6	57.6	842-1049 bp	1.0	64.3
atpF-atpH_p2	CCCAAAGAAAGGAAAGAATCGG	CCTGAGGCAGAGGGAAAAAT	45.5	50.0	57.6	57.5	846-1053 bp	1.0	63.5
atpF-atpH_p3	CCCAAAGAAAGGAAAGAATCGG	GCAGAGGGAAAAATACGGGG	45.5	55.0	57.6	58.6	840-1047 bp	1.0	62.8
atpF-atpH_p4	CCCAAAGAAAGGAAAGAATCGG	GGCAGAGGGAAAAATACGGG	45.5	55.0	57.6	58.6	841-1048 bp	1.0	62.8
atpF-atpH_p5	CCCAAAGAAAGGAAAGAATCGG	CAGAGGGAAAAATACGGGGT	45.5	50.0	57.6	57.2	839-1046 bp	1.0	61.3