Hotspot

trnR-UCU-atpA

LSC · 10414-10815 · 402 bp

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Pi 0.0585

MarkerSeek score 81.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 22
pi: 0.0585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTTACCGGGGAAGCAGAA	47.6	50.0	57.3	57.9	511-516 bp	1.0	72.9
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTTACCGGGGAAGC	47.6	50.0	57.3	57.9	515-520 bp	1.0	72.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	501-506 bp	1.0	70.9
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTTACCGGGGAAGCAGAAG	47.6	50.0	57.3	57.6	510-515 bp	1.0	70.5
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CATTTACCGGGGAAGCAGAA	47.6	50.0	56.9	57.9	546-551 bp	1.0	69.6