Hotspot

trnS-GCU-trnG-GCC

LSC · 8450-9291 · 842 bp

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Pi 0.0581

MarkerSeek score 85.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 46
pi: 0.0581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGTACCCC	50.0	50.0	58.0	57.2	954-955 bp	1.0	81.6
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGTACCCC	50.0	50.0	58.0	57.2	953-954 bp	1.0	81.6
trnS-GCU-trnG-GCC_p3	CAGATTAGCAATCCGACGCT	TGAATCAAACGAGGTACCCC	50.0	50.0	58.1	57.2	962-963 bp	1.0	80.5
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGTACCCC	50.0	50.0	58.2	57.2	1008-1009 bp	1.0	80.2
trnS-GCU-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	GAATCAAACGAGGTACCCCT	50.0	50.0	58.0	56.9	953-954 bp	1.0	79.2