Hotspot

ndhF

SSC · 113502-115754 · 2253 bp

Back to Prinsepia

Pi 0.0169

MarkerSeek score 54.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 56
pi: 0.0169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAATCGTCCGTTGCGATAT	AATAATCCAACCCACCTCGC	50.0	50.0	58.2	57.9	2993-2996 bp	0.666667	65.2
ndhF_p2	GCAGAATACCGTCACCCATT	TTATGGGTCAAGTTACGGGC	50.0	50.0	58.0	57.6	2891-2896 bp	0.666667	63.6
ndhF_p3	AAGAGGGATCCACCGAAGAA	TTATGGGTCAAGTTACGGGC	50.0	50.0	58.0	57.6	2768-2773 bp	0.666667	63.6
ndhF_p4	GCAGAATACCGTCACCCATT	TATGGGTCAAGTTACGGGCT	50.0	50.0	58.0	58.4	2890-2895 bp	0.666667	63.5
ndhF_p5	AGAGACCCGAGTGAATGGAA	TTATGGGTCAAGTTACGGGC	50.0	50.0	58.1	57.6	2686-2691 bp	0.666667	63.4