Hotspot

psbJ-psbL

LSC · 66840-66944 · 105 bp

Back to Prinsepia

Pi 0.0381

MarkerSeek score 68.70

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 6
pi: 0.0381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCCTTCCAGTAGTATCGGCC	ACACAATCAAACCCGAACGA	55.0	45.0	58.3	58.0	258-262 bp	1.0	80.3
psbJ-psbL_p2	TCCTTCCAGTAGTATCGGCC	ACGACACAATCAAACCCGAA	55.0	45.0	58.3	58.0	261-265 bp	1.0	80.3
psbJ-psbL_p3	TCCTTCCAGTAGTATCGGCC	CCCGAACGAACAAAGTGTTG	55.0	50.0	58.3	57.9	247-251 bp	1.0	80.0
psbJ-psbL_p4	TCCTTCCAGTAGTATCGGCC	AACCCGAACGAACAAAGTGT	55.0	45.0	58.3	58.3	249-253 bp	1.0	78.7
psbJ-psbL_p5	TCCTTCCAGTAGTATCGGCC	ACCCGAACGAACAAAGTGTT	55.0	45.0	58.3	58.3	248-252 bp	1.0	78.7