Hotspot

trnF-GAA-ndhJ

LSC · 51533-52187 · 655 bp

Back to Prinsepia

Pi 0.0422

MarkerSeek score 82.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 40
pi: 0.0422
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	794-914 bp	1.0	78.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	793-913 bp	1.0	78.0
trnF-GAA-ndhJ_p3	GAGGATGCTGTGTCGTGAAT	TGGATAGGATGGCCTTTACG	50.0	50.0	58.0	56.8	818-938 bp	1.0	77.8
trnF-GAA-ndhJ_p4	GAGGATGCTGTGTCGTGAAT	GGATAGGATGGCCTTTACGT	50.0	50.0	58.0	56.8	817-937 bp	1.0	77.8
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	758-878 bp	1.0	77.8