Hotspot

psbZ-trnG-GCC

LSC · 38433-38820 · 388 bp

Back to Prinsepia

Pi 0.056

MarkerSeek score 78.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 26
pi: 0.056
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	405-591 bp	1.0	85.3
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGAGGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.7	58.1	406-592 bp	1.0	85.3
psbZ-trnG-GCC_p3	GGGTTGGTCGGGTAACAAAA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.3	58.1	391-577 bp	1.0	85.2
psbZ-trnG-GCC_p4	GGGTTGGTCGGGTAACAAAA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.3	58.1	392-578 bp	1.0	85.2
psbZ-trnG-GCC_p5	TTTGCTTCTCCTGAGGGTTG	GAACCCGCATCTTCTCCTTG	50.0	55.0	57.7	58.6	401-587 bp	1.0	80.8