Hotspot

trnR-UCU-atpA

LSC · 10586-10703 · 118 bp

Back to Prinsepia

Pi 0.0508

MarkerSeek score 72.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 118
variable_sites: 9
pi: 0.0508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TTTACCGACGAAGCAGAAGC	47.6	50.0	57.3	58.6	221-358 bp	1.0	64.6
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	ACATTTACCGACGAAGCAGA	47.6	45.0	57.3	57.3	224-361 bp	1.0	63.2
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCA	47.6	45.0	57.3	57.3	226-363 bp	1.0	63.2
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	GACGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.8	215-352 bp	1.0	63.1
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.8	213-350 bp	1.0	63.1