Hotspot

psbA-trnK-UUU

LSC · 1527-1885 · 359 bp

Back to Prinsepia

Pi 0.0481

MarkerSeek score 70.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 19
pi: 0.0481
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCCAGTTACAAAAGCGACCC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.1	58.6	357-462 bp	1.0	81.9
psbA-trnK-UUU_p2	TCCAGTTACAAAAGCGACCC	ACTAGTTCCGGGTTCGAGTC	50.0	55.0	58.1	58.8	347-452 bp	1.0	80.3
psbA-trnK-UUU_p3	TCCAGTTACAAAAGCGACCC	GACTAGTTCCGGGTTCGAGT	50.0	55.0	58.1	58.8	348-453 bp	1.0	80.3
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	377-482 bp	1.0	80.0
psbA-trnK-UUU_p5	TCCAGTTACAAAAGCGACCC	ATCCGACTAGTTCCGGGTTC	50.0	55.0	58.1	58.9	352-457 bp	1.0	79.7