Hotspot

atpF

LSC · 12209-13462 · 1254 bp

Back to Primulina

Pi 0.00889

MarkerSeek score 53.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1254
variable_sites: 60
pi: 0.00889
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CAATACCATCGCCCACTTGA	TTTCCGTGGACTTGTCGTTT	50.0	45.0	58.0	58.0	1745-1895 bp	1.0	90.1
atpF_p2	CACCCGCCATTACTTCATCA	TTTCCGTGGACTTGTCGTTT	50.0	45.0	58.0	58.0	1781-1931 bp	1.0	89.9
atpF_p3	CACTTGAAGTACGGTACCGG	TTTCCGTGGACTTGTCGTTT	55.0	45.0	58.0	58.0	1732-1882 bp	0.954545	88.3
atpF_p4	CCACTTGAAGTACGGTACCG	TTTCCGTGGACTTGTCGTTT	55.0	45.0	58.0	58.0	1733-1883 bp	0.954545	88.3
atpF_p5	CACTTGAAGTACGGTACCGG	TAGGGCTTGCTTCTATCGGA	55.0	50.0	58.0	57.9	1967-2117 bp	0.954545	87.5