Hotspot

rpl32-trnL-UAG

SSC · 113341-114157 · 817 bp

Back to Primulina

Pi 0.0193
MarkerSeek score 76.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
817
variable_sites
79
pi
0.0193
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCATCGTTAAAAGCGTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.0 57.9 835-1004 bp 1.0 81.4
rpl32-trnL-UAG_p2 TTGTGCATCGTTAAAAGCGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.0 57.9 836-1005 bp 1.0 81.4
rpl32-trnL-UAG_p3 TGTGCATCGTTAAAAGCGTT AGCAGCGTGTCTACCAATTT 40.0 45.0 57.0 57.8 828-997 bp 1.0 81.0
rpl32-trnL-UAG_p4 TGTGCATCGTTAAAAGCGTT TTTAGGAGATGACATGCCGC 40.0 50.0 57.0 58.0 890-1059 bp 0.954545 79.7
rpl32-trnL-UAG_p5 TTGTGCATCGTTAAAAGCGT TTTAGGAGATGACATGCCGC 40.0 50.0 57.0 58.0 891-1060 bp 0.954545 79.7