Hotspot

petB-petD

LSC · 77212-78139 · 928 bp

Back to Primulina

Pi 0.0177

MarkerSeek score 82.70

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 79
pi: 0.0177
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	ACTTTTGTACTGCCGCTTCT	TGACCCATACCTTTTGCCAA	45.0	45.0	57.7	57.3	1109-1141 bp	1.0	81.5
petB-petD_p2	CACTTTTGTACTGCCGCTTC	TGACCCATACCTTTTGCCAA	50.0	45.0	57.7	57.3	1110-1142 bp	1.0	81.0
petB-petD_p3	ACACTTTTGTACTGCCGCTT	TGACCCATACCTTTTGCCAA	45.0	45.0	58.3	57.3	1111-1143 bp	1.0	80.9
petB-petD_p4	TGTACTGCCGCTTCTTACTG	TGACCCATACCTTTTGCCAA	50.0	45.0	57.6	57.3	1104-1136 bp	1.0	80.7
petB-petD_p5	GTACTGCCGCTTCTTACTGT	TGACCCATACCTTTTGCCAA	50.0	45.0	57.6	57.3	1103-1135 bp	1.0	80.7