Hotspot

clpP-psbB

LSC · 72944-73403 · 460 bp

Back to Primulina

Pi 0.0182

MarkerSeek score 70.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 460
variable_sites: 37
pi: 0.0182
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	541-564 bp	1.0	88.0
clpP-psbB_p2	CGCACTATACGTCAACCCAA	GGCCGGGATCATTCAATACA	50.0	50.0	58.0	57.7	573-596 bp	1.0	87.4
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	544-567 bp	1.0	86.1
clpP-psbB_p4	TCCTCTCCAGGAATTCGGAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	512-535 bp	1.0	86.1
clpP-psbB_p5	CGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	532-555 bp	1.0	85.9