Hotspot

trnT-GGU-trnL-UAA

LSC · 47319-47973 · 655 bp

Back to Primulina

Pi 0.0159
MarkerSeek score 74.50
Primer available yes
Label trnT-GGU-trnL-UAA

Feature Metadata

label_name
trnT-GGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
655
variable_sites
54
pi
0.0159
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 704-730 bp 1.0 87.0
trnT-GGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 736-762 bp 1.0 85.8
trnT-GGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 698-724 bp 1.0 85.7
trnT-GGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 736-762 bp 1.0 84.8
trnT-GGU-trnL-UAA_p5 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 730-756 bp 1.0 84.6