Hotspot

rps4-trnT-GGU

LSC · 46873-47246 · 374 bp

Back to Primulina

Pi 0.0297

MarkerSeek score 79.70

Primer available yes

Label rps4-trnT-GGU

Feature Metadata

label_name: rps4-trnT-GGU
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 53
pi: 0.0297
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-GGU_p1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	478-582 bp	1.0	87.6
rps4-trnT-GGU_p2	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.6	57.8	446-550 bp	1.0	86.8
rps4-trnT-GGU_p3	GATCACTTCCGGCTTTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	507-611 bp	1.0	85.3
rps4-trnT-GGU_p4	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	477-581 bp	1.0	85.3
rps4-trnT-GGU_p5	GTTAGTCCTGGTAAAGCCCC	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	57.6	57.5	486-590 bp	1.0	84.7