Hotspot

trnS-GCU-rps4

LSC · 45964-46266 · 303 bp

Back to Primulina

Pi 0.0272

MarkerSeek score 72.70

Primer available yes

Label trnS-GCU-rps4

Feature Metadata

label_name: trnS-GCU-rps4
feature_type: igs
region: LSC
length_bp: 303
variable_sites: 41
pi: 0.0272
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-rps4_p1	AGGCTTTTGTTTACCGAGGG	AGATAGTAAATGGGTCGGTT	50.0	40.0	58.1	53.4	396-419 bp	1.0	54.6
trnS-GCU-rps4_p2	AGGCTTTTGTTTACCGAGGG	AGATAGTAAATGGGTCGGTTT	50.0	38.1	58.1	54.3	396-419 bp	1.0	53.9
trnS-GCU-rps4_p3	CCGAGTGGTTTAAGGCGTAG	AGATAGTAAATGGGTCGGTT	55.0	40.0	58.4	53.4	433-456 bp	1.0	52.5
trnS-GCU-rps4_p4	GGCTTTTGTTTACCGAGGGT	AGATAGTAAATGGGTCGGTT	50.0	40.0	58.4	53.4	395-418 bp	1.0	52.3
trnS-GCU-rps4_p5	ATGTAGGAGAGATGGCCGAG	AGATAGTAAATGGGTCGGTT	55.0	40.0	58.4	53.4	448-471 bp	1.0	52.2