Hotspot

psaA-ycf3

LSC · 42322-43109 · 788 bp

Back to Primulina

Pi 0.0315

MarkerSeek score 82.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 173
pi: 0.0315
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	929-1015 bp	1.0	90.9
psaA-ycf3_p2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	911-997 bp	1.0	90.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	910-996 bp	1.0	90.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	892-978 bp	1.0	90.7
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	858-944 bp	1.0	89.9