Hotspot

trnT-GGU-psbD

LSC · 32099-33399 · 1301 bp

Back to Primulina

Pi 0.0164

MarkerSeek score 75.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 108
pi: 0.0164
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1452-1496 bp	1.0	87.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1455-1499 bp	1.0	86.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.1	57.8	1425-1469 bp	1.0	86.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.1	57.8	1428-1472 bp	1.0	86.1
trnT-GGU-psbD_p5	GCGACCCGTTGAGGAATTTA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1495-1539 bp	0.954545	83.3