Hotspot

trnE-UUC-trnT-GGU

LSC · 31448-32026 · 579 bp

Back to Primulina

Pi 0.0168

MarkerSeek score 75.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 61
pi: 0.0168
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CTCCGTGAAAGAGAGGTGTC	GAACCGATGACTTACGCCTT	55.0	50.0	57.6	58.0	557-792 bp	1.0	87.7
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	600-835 bp	0.954545	86.8
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	579-814 bp	0.954545	86.5
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	576-811 bp	0.954545	86.3
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	597-832 bp	0.954545	85.7