Hotspot

petN-psbM

LSC · 29427-30417 · 991 bp

Back to Primulina

Pi 0.0193
MarkerSeek score 72.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
991
variable_sites
84
pi
0.0193
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1004-1157 bp 1.0 81.9
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1002-1155 bp 1.0 81.9
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1000-1153 bp 1.0 81.4
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 998-1151 bp 1.0 81.4
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 1001-1154 bp 1.0 81.3