Hotspot

ycf1

SSC · 122344-127809 · 5466 bp

Back to Primula

Pi 0.0556

MarkerSeek score 78.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5466
variable_sites: 1996
pi: 0.0556
unique_haplotype_count: 103
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATTGGGGGCTTGGACTTTT	TCCCGTTTGCGCAAGTAATA	45.0	45.0	57.9	57.9	1128-1167 bp	0.330097	61.0
ycf1_p2	AATTGGGGGCTTGGACTTTT	AAGAAAGGAGAAAGCACGCA	45.0	45.0	57.9	58.0	1175-1214 bp	0.320388	61.0
ycf1_p3	AAATTGGGGGCTTGGACTTT	AAGAAAGGAGAAAGCACGCA	45.0	45.0	57.9	58.0	1176-1215 bp	0.291262	59.9
ycf1_p4	AAATTGGGGGCTTGGACTTT	TCCCGTTTGCGCAAGTAATA	45.0	45.0	57.9	57.9	1129-1168 bp	0.300971	59.8
ycf1_p5	AAACAAATTGGGGGCTTGGA	AAGAAAGGAGAAAGCACGCA	45.0	45.0	58.2	58.0	1180-1219 bp	0.281553	58.9