Hotspot

rps16

LSC · 5172-6247 · 1076 bp

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Pi 0.0492

MarkerSeek score 82.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1076
variable_sites: 382
pi: 0.0492
unique_haplotype_count: 98
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGTTCGATGCACTGTTGTCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	2091-2999 bp	0.980583	90.2
rps16_p2	TGTTCGATGCACTGTTGTCA	AGTCTTCCCTCCCATCAGTC	45.0	55.0	58.1	58.1	1664-1957 bp	0.456311	68.8
rps16_p3	TGTTCGATGCACTGTTGTCA	GTCTTCCCTCCCATCAGTCT	45.0	55.0	58.1	58.1	1663-1956 bp	0.456311	68.8
rps16_p4	TGTTCGATGCACTGTTGTCA	AGGACTCAAGTCTTCCCTCC	45.0	55.0	58.1	58.1	1672-1965 bp	0.349515	65.1
rps16_p5	TGTTCGATGCACTGTTGTCA	TCAAGTCTTCCCTCCCATCA	45.0	50.0	58.1	57.7	1667-1960 bp	0.349515	62.8