Hotspot

ndhF-rpl32

SSC · 111306-112166 · 861 bp

Back to Primula

Pi 0.0792

MarkerSeek score 82.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 861
variable_sites: 413
pi: 0.0792
unique_haplotype_count: 99
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGCCGGCAAATAATAGAAC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1891-2297 bp	0.864078	85.3
ndhF-rpl32_p2	GAAGAGGGAATTGTGCGGAT	ATCCTCTTCGGCAATTGACC	50.0	50.0	58.0	58.0	1779-2037 bp	0.300971	63.3
ndhF-rpl32_p3	ACAAACGCCTTTTGACAAGC	ATCCTCTTCGGCAATTGACC	45.0	50.0	58.1	58.0	1579-1837 bp	0.300971	63.1
ndhF-rpl32_p4	CAAACGCCTTTTGACAAGCA	ATCCTCTTCGGCAATTGACC	45.0	50.0	58.1	58.0	1578-1836 bp	0.300971	63.0
ndhF-rpl32_p5	GCGCCGGCAAATAATAGAAC	ATCCTCTTCGGCAATTGACC	50.0	50.0	58.0	58.0	1750-2008 bp	0.291262	63.0