Hotspot

psbE-petL

LSC · 64646-65846 · 1201 bp

Back to Primula

Pi 0.0678

MarkerSeek score 81.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1201
variable_sites: 523
pi: 0.0678
unique_haplotype_count: 101
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	1476-1899 bp	0.990291	91.1
psbE-petL_p2	ATGGAATTCCTTGTCGGCTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	1565-1988 bp	0.961165	90.0
psbE-petL_p3	AAAGGATCAAAACGGCCAGT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1590-2013 bp	0.932039	89.1
psbE-petL_p4	AAGGATCAAAACGGCCAGTT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	58.0	58.0	1589-2012 bp	0.932039	89.1
psbE-petL_p5	AAGGATCAAAACGGCCAGTT	ACACGGACTCCCACATAAGA	45.0	50.0	58.0	58.1	1176-1547 bp	0.242718	61.1