Hotspot

trnE-UUC-trnT-GGU

LSC · 30643-31285 · 643 bp

Back to Primula

Pi 0.0681

MarkerSeek score 86.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 297
pi: 0.0681
unique_haplotype_count: 97
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	495-977 bp	0.980583	89.9
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	436-918 bp	1.0	89.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	457-939 bp	0.990291	89.5
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	460-942 bp	0.980583	89.1
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	492-974 bp	0.980583	88.7