Hotspot

rpoB-trnC-GCA

LSC · 26420-27454 · 1035 bp

Back to Primula

Pi 0.0678
MarkerSeek score 83.50
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1035
variable_sites
485
pi
0.0678
unique_haplotype_count
101
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 GAAAATACCGGATCCCCACC TCTCGTGAGTCCGTATCGAA 55.0 50.0 58.0 58.0 1892-2192 bp 0.61165 76.2
rpoB-trnC-GCA_p2 CCTACGCAGGCAAATTGTTG TCTCGTGAGTCCGTATCGAA 50.0 50.0 58.0 58.0 1874-2068 bp 0.359223 66.1
rpoB-trnC-GCA_p3 CCTACGCAGGCAAATTGTTG ATCGAAATTCTGCCCCTTCC 50.0 50.0 58.0 57.9 1860-2051 bp 0.223301 60.3
rpoB-trnC-GCA_p4 CCTACGCAGGCAAATTGTTG TTCTGCCCCTTCCATCACTA 50.0 50.0 58.0 58.0 1853-2039 bp 0.184466 59.0
rpoB-trnC-GCA_p5 GAAAATACCGGATCCCCACC TTCTGCCCCTTCCATCACTA 55.0 50.0 58.0 58.0 1871-2134 bp 0.15534 57.8