Hotspot

atpH-atpI

LSC · 13438-14167 · 730 bp

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Pi 0.0637

MarkerSeek score 83.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 303
pi: 0.0637
unique_haplotype_count: 97
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATCAGTCCTTCCCATCCCA	GACTGGCGGTCAGAATTTCT	50.0	50.0	58.0	57.9	1581-1725 bp	0.883495	86.4
atpH-atpI_p2	AATCAGTCCTTCCCATCCCA	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1061-1205 bp	0.873786	86.3
atpH-atpI_p3	AATCAGTCCTTCCCATCCCA	TTCGGAACCCACAAACCATT	50.0	45.0	58.0	57.9	1645-1733 bp	0.543689	73.0
atpH-atpI_p4	AATCAGTCCTTCCCATCCCA	AATTGGGGAAGAATACGGCC	50.0	50.0	58.0	57.9	1584-1662 bp	0.504854	71.5
atpH-atpI_p5	AATCAGTCCTTCCCATCCCA	TCTTATTGGGTTCAGCCACG	50.0	50.0	58.0	57.9	1696-1752 bp	0.300971	62.8