Hotspot

trnS-GCU-trnG-UCC

LSC · 8210-8813 · 604 bp

Back to Primula

Pi 0.0758

MarkerSeek score 83.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 298
pi: 0.0758
unique_haplotype_count: 98
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCATCCACTCAGCCATCTCT	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	446-773 bp	0.990291	62.0
trnS-GCU-trnG-UCC_p2	GCTTTCATCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	450-777 bp	1.0	61.8
trnS-GCU-trnG-UCC_p3	TCATCCACTCAGCCATCTCT	AGAACGAATCACACTTTTACCAC	50.0	39.1	57.8	57.3	449-776 bp	0.990291	60.9
trnS-GCU-trnG-UCC_p4	TCATCCACTCAGCCATCTCT	GAACGAATCACACTTTTACCACT	50.0	39.1	57.8	57.3	448-775 bp	0.990291	60.9
trnS-GCU-trnG-UCC_p5	GCTTTCATCCACTCAGCCAT	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	452-779 bp	1.0	60.7