Hotspot

rps16-trnQ-UUG

LSC · 6248-6960 · 713 bp

Back to Primula

Pi 0.0884
MarkerSeek score 84.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
713
variable_sites
364
pi
0.0884
unique_haplotype_count
100
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 733-1506 bp 0.990291 91.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 726-1499 bp 1.0 90.3
rps16-trnQ-UUG_p3 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 734-1507 bp 0.980583 87.8
rps16-trnQ-UUG_p4 GTCTTTCAAGTCGCACGTTG TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.0 57.3 737-1510 bp 0.990291 86.2
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 730-1503 bp 1.0 85.5