Hotspot

trnK-UUU-rps16

LSC · 4362-5171 · 810 bp

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Pi 0.0816
MarkerSeek score 80.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
810
variable_sites
404
pi
0.0816
unique_haplotype_count
101
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCAGGATCAGTCGTGGTCTT CGAGATCTTCGGAAAGTGGG 50.0 55.0 58.1 58.1 859-1155 bp 0.893204 86.5
trnK-UUU-rps16_p2 TTCAGGATCAGTCGTGGTCT CGAGATCTTCGGAAAGTGGG 50.0 55.0 58.1 58.1 860-1156 bp 0.893204 86.5
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT TTTGAATGAGCCGTACGAGG 50.0 50.0 58.1 58.0 983-1279 bp 0.38835 66.9
trnK-UUU-rps16_p4 TTCAGGATCAGTCGTGGTCT TTTGAATGAGCCGTACGAGG 50.0 50.0 58.1 58.0 984-1280 bp 0.38835 66.9
trnK-UUU-rps16_p5 AGTATTTCTCGCACCCACAC TTTGAATGAGCCGTACGAGG 50.0 50.0 57.9 58.0 1251-1519 bp 0.31068 63.6