Hotspot

trnH-GUG-psbA

LSC · 90-510 · 421 bp

Back to Primula

Pi 0.128

MarkerSeek score 72.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 258
pi: 0.128
unique_haplotype_count: 96
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CTGCGACTGCTGTTTTCTTG	50.0	50.0	57.9	58.0	794-1102 bp	0.980583	89.9
trnH-GUG-psbA_p2	TTAGTTATGGGCGAACGACG	CTGCGATTGAAGCTCCATCT	50.0	50.0	58.1	58.0	345-563 bp	0.495146	70.7
trnH-GUG-psbA_p3	TTAGTTATGGGCGAACGACG	GGTGTAGCTGGTGTATTCGG	50.0	55.0	58.1	58.1	775-993 bp	0.495146	70.6
trnH-GUG-psbA_p4	TTAGTTATGGGCGAACGACG	CTGCGACTGCTGTTTTCTTG	50.0	50.0	58.1	58.0	918-1136 bp	0.485437	70.5
trnH-GUG-psbA_p5	TTAGTTATGGGCGAACGACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	361-579 bp	0.485437	70.1