Hotspot

ndhD

SSC · 116781-118283 · 1503 bp

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Pi 0.00355
MarkerSeek score 36.80
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
9
pi
0.00355
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TTGGAGTTGGGACCCAAAAG GATACCCTGGGACGGATGTA 50.0 55.0 57.9 58.0 2270-2286 bp 1.0 81.5
ndhD_p2 GAAGCGTGGGGATCCTATTG GATACCCTGGGACGGATGTA 55.0 55.0 58.1 58.0 2287-2303 bp 1.0 81.2
ndhD_p3 TTGGAGTTGGGACCCAAAAG AAATAGCTTCCGCTCCAAGG 50.0 50.0 57.9 58.0 2241-2257 bp 1.0 81.2
ndhD_p4 CTCTCGCCAGTTCCAATTCA GATACCCTGGGACGGATGTA 50.0 55.0 57.9 58.0 1926-1945 bp 1.0 81.0
ndhD_p5 CTCTCGCCAGTTCCAATTCA AAATAGCTTCCGCTCCAAGG 50.0 50.0 57.9 58.0 1897-1916 bp 1.0 80.7