Hotspot

trnL-UAG-ccsA

SSC · 115444-115540 · 97 bp

Back to Premna

Pi 0.0295
MarkerSeek score 62.20
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
97
variable_sites
4
pi
0.0295
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.81
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TTGAAACGATCGAAAAAGAAA 50.0 28.6 57.9 52.6 198-216 bp 1.0 45.6
trnL-UAG-ccsA_p2 GGTAGACACGCTGCTCTTAG TTGAAACGATCGAAAAAGAAA 55.0 28.6 57.8 52.6 200-218 bp 1.0 45.6
trnL-UAG-ccsA_p3 GTAGACACGCTGCTCTTAGG TTGAAACGATCGAAAAAGAAA 55.0 28.6 57.8 52.6 199-217 bp 1.0 45.6
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TTGAAACGATCGAAAAAGAAA 45.0 28.6 57.8 52.6 205-223 bp 1.0 45.5
trnL-UAG-ccsA_p5 ACAAGCCGCTATGGTGAAAT TTGAAACGATCGAAAAAGAAA 45.0 28.6 57.9 52.6 221-239 bp 1.0 45.4