Hotspot

atpB-rbcl

LSC · 56226-57015 · 790 bp

Back to Premna

Pi 0.00679
MarkerSeek score 64.80
Primer available yes
Label atpB-rbcl

Feature Metadata

label_name
atpB-rbcl
feature_type
igs
region
LSC
length_bp
790
variable_sites
9
pi
0.00679
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcl_p1 GATGATACGCCCCAGGTTTT GCCTTGAATCCAACACTTGC 50.0 50.0 57.9 57.9 906-928 bp 1.0 81.1
atpB-rbcl_p2 GGAAACCCCAGAACCAGAAG GCCTTGAATCCAACACTTGC 55.0 50.0 58.1 57.9 873-895 bp 1.0 80.9
atpB-rbcl_p3 GATGATACGCCCCAGGTTTT ACTCTTTAACACCCGCCTTG 50.0 50.0 57.9 58.1 920-942 bp 1.0 80.8
atpB-rbcl_p4 GGAAACCCCAGAACCAGAAG ACTCTTTAACACCCGCCTTG 55.0 50.0 58.1 58.1 887-909 bp 1.0 80.6
atpB-rbcl_p5 GATGATACGCCCCAGGTTTT TTAACACCCGCCTTGAATCC 50.0 50.0 57.9 58.2 915-937 bp 1.0 80.3