Hotspot

trnF-GAA-ndhJ

LSC · 49778-50459 · 682 bp

Back to Premna

Pi 0.0128
MarkerSeek score 67.00
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
682
variable_sites
15
pi
0.0128
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 790-824 bp 1.0 84.2
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 754-788 bp 1.0 84.0
trnF-GAA-ndhJ_p3 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 55.0 50.0 57.9 58.1 778-812 bp 1.0 83.2
trnF-GAA-ndhJ_p4 GAATGGTCGGGATAGCTCAG AGTTGGATAGGATGGCCCTT 55.0 50.0 57.6 58.1 798-832 bp 1.0 80.6
trnF-GAA-ndhJ_p5 GGGATAGCTCAGCTGGTAGA TGATAATCATCCGCGCTTGA 55.0 45.0 58.0 57.5 830-864 bp 1.0 80.5