Hotspot

trnT-UGU-trnL-UAA

LSC · 48089-48817 · 729 bp

Back to Premna

Pi 0.0124
MarkerSeek score 65.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
729
variable_sites
15
pi
0.0124
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 745-790 bp 1.0 82.5
trnT-UGU-trnL-UAA_p2 TAGGAAAAAGCCGGCTATCG CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 796-841 bp 1.0 81.6
trnT-UGU-trnL-UAA_p3 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 777-822 bp 1.0 81.6
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 739-784 bp 1.0 81.2
trnT-UGU-trnL-UAA_p5 TAGGAAAAAGCCGGCTATCG CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 790-835 bp 1.0 80.3