Hotspot

atpH-atpI

LSC · 14423-15447 · 1025 bp

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Pi 0.0166
MarkerSeek score 74.80
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1025
variable_sites
30
pi
0.0166
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.1 58.5 1111-1125 bp 1.0 81.2
atpH-atpI_p2 AGCCAATCCAGCAGCAATAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 57.9 58.5 1129-1143 bp 1.0 80.8
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.8 58.5 1128-1142 bp 1.0 80.3
atpH-atpI_p4 AACGGAAGCAGCAGAAATCA TCAGTGGTTCCTATCCCTGT 45.0 50.0 58.1 57.4 1172-1186 bp 1.0 80.1
atpH-atpI_p5 TCCAGCAGCAATAACGGAAG TTTTTGCAACTTTAGCCGCG 50.0 45.0 58.3 58.5 1123-1137 bp 1.0 79.9