Hotspot

atpA-atpF

LSC · 12443-12534 · 92 bp

Back to Premna

Pi 0.0214
MarkerSeek score 63.20
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
92
variable_sites
3
pi
0.0214
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.74
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 ACTTGAAGTACGGTACCGGT ATTGGCATGTTGGGAACGAT 50.0 45.0 58.4 58.1 210-235 bp 1.0 80.9
atpA-atpF_p2 TCGCCTACTTGAAGTACGGT ATTGGCATGTTGGGAACGAT 50.0 45.0 58.5 58.1 216-241 bp 1.0 80.2
atpA-atpF_p3 ACTAATTTCGTCGGCTCGAA ATTGGCATGTTGGGAACGAT 45.0 45.0 57.4 58.1 136-161 bp 1.0 79.3
atpA-atpF_p4 ACTTGAAGTACGGTACCGGT TATTGGCATGTTGGGAACGA 50.0 45.0 58.4 57.5 211-236 bp 1.0 78.1
atpA-atpF_p5 TCGCCTACTTGAAGTACGGT TATTGGCATGTTGGGAACGA 50.0 45.0 58.5 57.5 217-242 bp 1.0 77.4